hfob1 19 cells  (ATCC)

Journal: Translational Cancer Research

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

doi: 10.21037/tcr-2025-aw-2290

Figure Lengend Snippet: The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.

Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal human osteoblast hFOB1.19 (RRID: CVCL_3889), were sourced from Servicebio company (Wuhan, China).

Techniques: Expressing, CCK-8 Assay, Transwell Migration Assay, Migration, Transfection, Staining, Cell Counting, Flow Cytometry, Negative Control

Journal: Translational Cancer Research

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

doi: 10.21037/tcr-2025-aw-2290

Figure Lengend Snippet: The expression of FBXW7 in osteosarcoma cells. (A) The mRNA levels of FBXW7 in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2, MG-63 were also analyzed. (B,C) To explore the impact of FBXW7 on the biological behavior of osteosarcoma cells Saos-2 and MG-63, the researchers performed transfections on these two osteosarcoma cell lines using pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7. (D,E) Effect of transfection of pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7 on expression of FBXW7 and β-catenin in osteosarcoma cells MG-63 (D) and Saos-2 (E). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. NC, negative control; OE, overexpression.

Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal human osteoblast hFOB1.19 (RRID: CVCL_3889), were sourced from Servicebio company (Wuhan, China).

Techniques: Expressing, Transfection, Negative Control, Over Expression

Journal: bioRxiv

Article Title: A DNA foundation model predicts osteoporosis risk genes without proximity bias

doi: 10.64898/2026.03.09.707383

Figure Lengend Snippet: a. All variant–gene pairs within a receptive field of 256kbp are scored by Rosalind. Genes above the elbow threshold are kept as candidate causal genes. b. The distribution of protein-coding genes around the 1103 lead variants from the eBMD GWAS (Morris et al. ) when using a window of 256kbp. c. Overlap of Rosalind-predicted genes with nearest genes is shown, with genes known to regulate eBMD highlighted. d. Mineralization assay experimental workflow: arrayed CRISPR/Cas9 knock-out screen of hFOB1.19 human osteoblasts. e. Plot shows SNP RSID-gene pairs, where at least one hit was observed in the osteoblast mineralisation assay. Hit calling was performed using Region of Practical Equivalence (ROPE) analysis and results show hits at ROPE 0.5 or greater (‘moderate’ effect size) f. Experimental validation summary, with assay “hits” represented in blue and “no effect” represented in red.

Article Snippet: hFOB1.19 (ATCC) were cultured in flasks in DMEM/F-12, 10% FBS (Sigma F7524 lot.0001663052), antibiotic/antimycotic (Gibco 15240-062) and G418 sulfate (ab144261) at 34oC until 80-90% confluent.

Techniques: Variant Assay, Mineralization Assay, CRISPR, Knock-Out, Biomarker Discovery

Journal: Molecular Cancer

Article Title: METTL3-mediated m 6 A modification of CACNA1E promotes osteosarcoma progression and chemoresistance by enhancing WNT7B-mediated Ca 2+ signaling

doi: 10.1186/s12943-025-02553-x

Figure Lengend Snippet: Identification of CACNA1E as an m 6 A-hypermethylated and upregulated gene in OS. A Starplot illustrating the distribution of genes with differential expression (up/down; |log2fold change|≥ 1 and P < 0.05) and differential m 6 A (hyper/hypo; |log2fold change|≥ 1 and P < 0.05) in OS tissues with adjacent normal tissues. B Heatmap showing the top5 up-/down-regulated genes in OS tissues versus adjacent normal tissues. C CACNA1E mRNA level examined by qRT-PCR in OS and adjacent normal tissues. D CACNA1E mRNA level examined by qRT-PCR in osteoblast cells (hFOB1.19) and OS cells (SJSA-1, MNNG, U2OS, and SAOS2). E Kaplan–Meier survival curves of OS patients ( n = 85) stratified by high and low CACNA1E expression, with log-rank test for comparing survival rate. F Multivariate Cox regression analysis for determining CACNA1E as an independent prognostic factor after adjusting for clinical variables (age, gender, and metastasis status) based on the TARGET database. G - I qRT-PCR and Western blot assays for verifying the stable knockdown of CACNA1E in MNNG and SJSA-1 cells. J , K CCK-8 and ( L , M ) colony formation assays for determining the effect of CACNA1E on OS cell proliferation. N , O Wound healing assay for examining the migration of shNC- and shCACNA1E-transfected OS cells. Photographs are displayed at 0, 6, 12, and 24 h. Scale bar, 100 μm. P - R Analysis of OS cell migration and invasion by Transwell assays. Scale bar, 100 μm. S - U Western blot for examining the effect of CACNA1E on EMT markers in OS cells. These data are presented as the means ± SD of three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 derived from Student’s t-test or one- or two-way analysis of variance

Article Snippet: Human 293 T (HTX1559), SJSA-1 (HTX2137), MNNG (HTX1631), and U2OS (HTX1634) cell lines were obtained from Otwo Biotech (ShenZhen, China), and hFOB1.19 (CL-0353) and SAOS2 (CL-0202) cell lines were acquired from Procell (Wuhan, China).

Techniques: Quantitative Proteomics, Quantitative RT-PCR, Expressing, Western Blot, Knockdown, CCK-8 Assay, Wound Healing Assay, Migration, Transfection, Derivative Assay

Journal: Molecular Cancer

Article Title: METTL3-mediated m 6 A modification of CACNA1E promotes osteosarcoma progression and chemoresistance by enhancing WNT7B-mediated Ca 2+ signaling

doi: 10.1186/s12943-025-02553-x

Figure Lengend Snippet: CACNA1E facilitates OS progression through modulating WNT7B expression. A Volcano plots for differentially expressed transcripts between shCACNA1E- and shNC-transfected OS cells based on RNA-seq data. B KEGG enrichment plots for signaling pathways enriched by the differentially expressed transcripts. C Proteins interacting with WNT7B and CACNA1E using the GeneMANIA database. D CACNA1E and WNT7B mRNA levels in OS and normal tissues. E WNT7B mRNA level examined by qRT-PCR in osteoblast cells (hFOB1.19) and OS cells (SJSA-1, MNNG, U2OS, SAOS2). F – H WNT7B mRNA and protein levels detected by qRT-PCR and Western blot in shCACNA1E- and shNC-transfected OS cells. I WNT7B mRNA level examined by qRT-PCR in shMETTL3- and shNC-transfected OS cells. J , K Cell viability of METTL3-knockdown or/and WNT7B-overexpressing OS cells determined by CCK-8. L , M Colony-forming potential of OS cells. N , O Migratory ability of OS cells assessed by wound healing assay. Photographs are displayed at 0, 6, 12, and 24 h. Scale bar, 100 μm. P - R Migratory and invasive potential of OS cells measured by Transwell assays. Scale bar, 100 μm. S , T Kaplan–Meier plots of overall survival and disease-free survival in OS patients stratified by WNT7B expression. These data are presented as the means ± SD of three independent experiments. ** P < 0.01; *** P < 0.001; **** P < 0.0001 derived from one- or two-way analysis of variance

Article Snippet: Human 293 T (HTX1559), SJSA-1 (HTX2137), MNNG (HTX1631), and U2OS (HTX1634) cell lines were obtained from Otwo Biotech (ShenZhen, China), and hFOB1.19 (CL-0353) and SAOS2 (CL-0202) cell lines were acquired from Procell (Wuhan, China).

Techniques: Expressing, Transfection, RNA Sequencing, Protein-Protein interactions, Quantitative RT-PCR, Western Blot, Knockdown, CCK-8 Assay, Wound Healing Assay, Derivative Assay